Incubate 2 h at RT. 96-well culture plateFOR HTRF CERTIFIED READER For more information about HTRF. (2) Forskolin activates the adenylyl cyclase enzyme and increases the intracellular level of cAMP. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. Microplate reader assays requiring infrared (IR) or. FOR HTRF CERTIFIED READER For more. Lysates are then transferred to the assay plate for the detection of total-CDK4 by HTRF. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Detection plates from providers not listed on this. If not, our scientific team will help you choose the instrument that best suits your needs and budget. For more information about HTRF. Incubate 24 hours at RT. Lysates are then transferred to the assay plate for the detection of total-NFkB by HTRF. HTRF reagent concentrations have been set for optimal assay performances. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate 25 µL cells + 5 µL compounds + 10 µL supplemented lysis buffer (4X)* + 4 µL HTRF® pre-mixed antibodies Read & analyse Incubate 4h at RT Transfer 16 µL supernatant LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . Lyse cells Read on an HTRF compatible reader . Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. • HTRF®-Certified Reader. In particular, these parameters should be entered as below. FOR HTRF CERTIFIED READER. If not, our scientific team will help you choose the instrument that best suits your needs and budget. FOR HTRF CERTIFIED READER. Read & analyse. Plate 25 μL of cells in. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. The kit includes everything you need: reagents, buffers and a microplate. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the. Plate cells Stimulate cells 3. Remove the plate seal and read on an HTRF compatible reader. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Live Viewer for cell-based assays. It also integrates with the powerful SoftMax Pro Software for. For HTRF certified readerThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Lysis buffer 1X for assay protocol on tissues, two-plate assay protocol on adherent cells, and standard dilution. Typical applications The HTRF cartridge provides optimal performance for key applications. FOR HTRF CERTIFIED READER. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed TNFα Antibodies ADD READ ANALYSE Incubate 2 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. * For HTRF microplate recommendations, please visit . For a list of HTRF-compatible readers and set-up recommendations, please visit . Revvity. Remove the plate sealer and read on an HTRF® compatible reader . FOR HTRF CERTIFIED READER. Our microplates and accessories are manufactured under strict process controls guaranteeing consistent product performance for. Dual-Luciferase® (DLR™) gene assays. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance forCisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Set up your reader for Eu3+ Cryptate and read the fluorescence emission at two different wavelengths (665nm and 620nm) on a compatible HTRF® reader**. For more. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. FOR HTRF CERTIFIED READER For more. At Cisbio we provide microplates that help you discover better. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Read & analyse. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Nephelometers are dedicated instruments that can only detect scattering and are only available as standalone plate readers. Other technologies such as imaging. These plates can be read on all HTRF certified readers, thus making their implementation in. Dedicated UV-laser excitation. FOR HTRF CERTIFIED READER For. 1. Plate 25 μL of cells in. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. Rating: 5. Some vendors hold the entire market share for not only a. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Incubate overnight at RT. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERHTRF Ratio Anti-Tag Antibody Cryptate Inhibitor-Red Inhibitor Purified Tagged Kinase Figure 2: Principle of HTRF kinase competition binding assay (IC 50 - KI determination) HTRF Dasatinib-Red Part # 62KB02REDC & 62KB02REDE Amount: 1 nmol (62KB02REDC) & 20 nmol (62KB02REDE) Concentration: 25 µM in DMSO Form:. This is thanks to a dedicated Alpha Technology laser which outperforms any xenon-based light source by exciting donors at 680 nm. 2. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance forThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit. EX: HTRF Emission range. HTRF is a proprietary time-resolved fluorescence technology that overcomes Results summary for HTRF IP1 standard curve. FOR HTRF CERTIFIED READER For. Step 1 Step 2 Step 3 Step 4 Step 5. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. These plates can be read on all HTRF certified readers, thus making their implementation in. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. 25 µL cells + 5 µL compounds + 10 µL supplemented lysis buffer (4X)* + 4 µL HTRF ® pre-mixed antibodies. FOR HTRF CERTIFIED READER. This protocol, HTS designed, allows. certified for use with HTRF. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Discover our high performance reader. acceptor reagent. Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. An HTRF assay measures the fluorescence energy transfer between molecules in close proximity. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. PerkinElmer’s 96w low volume, 96w and 384w microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. FOR HTRF CERTIFIED READER culture plate to a 384-well small volume white plate. According to the manufacturers protocol, this protein assay is linear in the range of 0. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. FOR HTRF CERTIFIED READER. Reagents were dispensed following Cisbio’s ligand binding protocol (Figure 3). For fluorescent assays, black plates absorb light and reduce background and crosstalk. Cell seeding densities of 2,000,000 cells/well are generally sufficient for most cell lines, but. Lyse cells Read on an HTRF compatible reader . Plate 25 μL of cells in. Unlike standard ELISA plate readers, MSD. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Preconfigured HTRF protocols in SoftMax ® Pro Software 7. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of Total Cereblon HTRF detection reagents. 8 µL cells. It features a modular design that allows users to add or change detection cartridges according to their needs. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. 27 / Pack of 100; Qty. At Cisbio we provide microplates that help you discover better. For more information about HTRF. Plate 25 μL of cells in. If not, our scientific team will help you choose the instrument that best suits your needs and budget. High Speed Monochromator offers a typical wavelength accuracy of <0. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. 55 µL anti-cAMP-Cryptate. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. The system provides even higher sensitivity for time-resolved fluorescence (TRF) applications. It is used as a positive control for Gi applications ( biological models validation,FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER For. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. For more information about HTRF. Incubate overnight at RT. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. No washing steps are required. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. 96-well culture plate• HTRF®-Certified Reader. Choosing the right microplate reader ensures you’ll get an optimal readout. Designed to the highest standards to support your drug discovery research, Corning ® and Falcon ® microplates are ideal for drug discovery applications, including assay prep, assay development, and HTS and automation. 96-well culture plateFOR HTRF CERTIFIED READER. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detectionThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. HTRF is compatible with a large range of available microplate readers. The lysis buffer 4X is ready-to-use. 5 µL. FOR HTRF CERTIFIED READER For. For more. The FLUOstar ® Omega is a multi-mode microplate reader with three detection modes. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. For a list of HTRF-compatible readers and set-up recommendations, please visit . Assay plate setup. The assay is then ready to be read on an HTRF compatible or certified microplate reader. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations. The Spark multimode reader from Tecan performs accurate and sensitive measurements of fluorescent cell-based assays. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SV detection plate. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Due to the chemical and photophysical. Already more than 50 certified or compatible instruments, and counting! HTRF is compatible with a large range of available microplate readers and certainly the detection system already in your lab. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. VICTOR Nivo Multimode Plate Reader. FOR HTRF CERTIFIED READER. EGFR protein (PV3872, 0. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 10 µL Standard or Sample. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. For more information. FOR HTRF CERTIFIED READER For more information about HTRF. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Plate 25 μL of cells in. MATERIALS PROVIDED: KIT . 96-well or 384-well small volume (SV) detection. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 1) . Manufacturer. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Make sure the setup for Eu Cryptate is used. For more information about HTRF. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. 3. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Make sure the setup for Eu 3+ Cryptate is used. VICTOR® Nivo™ multimode plate reader. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. MATERIALS PROVIDED: KIT . FOR HTRF CERTIFIED READER. Read & analyse. Plate 25 μL of cells in. The detection antibody is incubated for 1. For more information about HTRF ®Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. February 23, 2010, WINOOSKI VT, USA - The Synergy TM H4 Hybrid Microplate Reader from BioTek Instruments is now HTRF® certified. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. Incubate 4h at RT. Customer Transformation Hub. FOR HTRF CERTIFIED READER. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersHTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. EM1 616/10 nm EM2 665/10 nm Certified for use with Cisbio’s HTRF Europium Cryptate reagents. When equipped with a quad monochromator, the EnSight plate reader can improve performance for absorbance and fluorescence detection. Read & analyse. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. The PHERAstar FSX is the best microplate reader for all HTRF assays: Sensitive, fast and reliable measurements with best Z´ and DeltaF% HTRF-certified with white and black. The HTRF ratio obtained represents the total binding. + 4 µL HTRF pre-mixed antibodies. On the SpectraMax i3x reader, install the HTRF cartridge, and wavelengths will be selected automatically when HTRF is selected as the Optical Configuration. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. Lysates are then transferred to the assay plate for the detection of phospho- CHK2 (Thr 68) by HTRF. Lysates are then transferred to the assay plate for the detection of total-CREB by HTRF. Make sure the setup for Eu3+ Cryptate is used. Concentrate on running your assays instead of running your instrument: the optic modules contain all the filters, dichroics, and more needed to run your assay. Plate 25 μL of cells in. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. (In the following text, the term YTHDC1 is used frequently as an abbreviation for the m 6 A-recognition domain of YTHDC1). a one- plate assay protocol. Measure elevated and normal expression samples without multiple dilutions. FOR HTRF CERTIFIED READER. Plate 25 μL of cells in. Lysates are then transferred to the assay plate for the detection of total-STING by HTRF. 1. Revvity. For more information about HTRF. + 4 µL HTRF ® pre-mixed antibodies . These plates can be read on all HTRF certified readers, thus making their implementation in. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. Lysis buffer 1X for two-plate assay protocol on adherent cells Determine the amount of lysis buffer needed for the experiment. In this case the Z´(pronounced Z prime) value comes to mind. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. For a list of HTRF-compatible readers and set -up recommendations, please visit. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. The ratio between control lysate signal / non-specific signal should be greater than 2. Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to. Make sure the setup for Tb Cryptate is used. After defining the objectives and their locations in Gen5: 1. Read & analyse. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader. Use simpler, faster protocols. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. FOR HTRF CERTIFIED READER For more. Reagents were dispensed following Cisbio’s ligand binding protocol in 384-well format (white plates). PLATE READER INTRODUCTION Selecting the right plate reader for your lab can be challenging. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. It fits any benchtop – and budget Building on our pioneering family of benchtop multimode plate readers, the VICTOR® Nivo™ multimode reader breaks new ground, packing all popular detection modes into the industry’s smallest benchtop. FOR HTRF CERTIFIED READER. For more information about HTRF ®Excitation range. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. STANDARD . HTRF Signal + 2 hours @ RT [Cortisol] ng/ml 10 µL Standard or Sample 5 µL Cortisol acceptor reagent 5 µL Cortisol donor antibody ADD READ & ANALYSESafire 2™ readers must be appropriately be configured for HTRF readout by setting up the measurement conditions in the “multilabeling” function of Magellan software. As shown in Fig. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. PROTOCOL AT A GLANCE Do not pre-mix the d2 and Cryptate solutions prior to dispensing. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Better sensitivity provides better statistic, less variability among replicates, and a larger assay window. 96-well culture plateONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. It continues to deliver optimal results. Plate cells Stimulate cells 3. support such assays and even feature endorsements from reagent suppliers saying the reader is “HTRF compatible,” “LanthaScreen Certified Plus,” Transcreener Validated” or. This protocol enables the cells' viability and confluence to be monitored. Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. FOR HTRF CERTIFIED READER For more information about HTRF. HTRF-compatible SpectraMax Multi-Mode Readers. Incubate 4h at room temperature. PROTOCOL AT A GLANCE. Make sure the setup for Tb3+ Cryptate is used. It also integrates with the. For more information. The Dual-Luciferase ® Reporter Assay or DLR is widely used to study gene transcription and regulation. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. SERIAL DILUTIONS . FOR HTRF CERTIFIED READER. EnVision Nexus ultimode Plate Reader 2 Benefits • Provides the speed and sensitivity to boost your lab’s productivity with ultra-high throughput • Freely configurable and upgradable to adapt to your needs today and tomorrow • Fully automatable for walkaway convenience • Certified for use with HTRF® technology Key featuresBMG LABTECH plate readers certified for LanthaScreen detection include the PHERAstar FSX, CLARIOstar Plus and VANTAstar. It can also be further streamlined to a one-. The mean and standard deviation. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:FOR HTRF CERTIFIED READER. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERHTRF® 96-well low volume plate TECHNOLOGY ALL THE BENEFITS OF HTRF WITH THE EASE OF USE OF AN ELISA PLATE!. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. It combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for excellence. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. and Canaa -8-963-4567. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Discover our portfolio of multimode plate readers and radiometric detection solutions, complemented by assays, reagents and consumables which together enable you to increase your lab’s. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. CLARIOstar is equipped with a specific optical device which enables the measurement of. Evaluation of the performance of the Spark™ multimode reader for far red fluorescence emission. Lysates are then transferred to the assay plate for the detection of total cRaf by HTRF. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. overnight at 4 C [Prostaglandin E2] pg/ml. Phospho-Rb (Ser780) two-plate assay protocol. LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Remove the plate seal and read on an HTRF compatible reader. Finding a plate reader that offers compatibility with microplates sold by multiple leading vendors can protect both the long-term cost and the performance of assays. SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. FOR HTRF CERTIFIED READER. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. + 4 µL HTRF pre-mixed antibodies. The ratio for each well must be calculated individually. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. no. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. . It utilises an ultra-fast UV/vis spectrometer or filters for absorbance as well as highly sensitive filters for all other detection modes. Remove the plate sealer and read on an HTRF® compatible reader . The EnSight<sup>®</sup> multimode plate reader offers HTS Alpha and Ultra-sensitive Luminescence technologies alongside conventional multimode detection technologies. For more information. This protocol gives the cells viability and confluence to be monitored. Assay detection requires a microplate reader with. * For HTRF microplate recommendations, please visit . Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. This protocol gives the cells viability and confluence to be monitored. FOR HTRF CERTIFIED READER. Dual monochromators for assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. For HTRF readings, a 620/40 nm filter (PerkinElmer 2100-5760) and a 665/7. PURCHASE SEPARATELY . The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The SpectraMax i3x and SpectraMax iD5 readers can be equipped with an HTRF-certified detection cartridge (i3x) or an HTRF-certified module and filters (iD5) to meet the demanding specifications required for HTRF assays. HTRF certified . Plate 25 μL of cells in. These plates can be read on all HTRF certified readers, thus making their implementation in. 1. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. Incubate at room temperature. The EnVision XCite Plate Reader provides exceptional sensitivity and speed with minimal downtime - backed by our global network of application scientists and engineers. The VICTOR Nivo is a HTRF certified microplate reader. 96-well culture plateHTRF certified . The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same . FOR HTRF CERTIFIED READER. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. Incubate overnight at RT. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. For more ® information about HTRF compatible readers and for set-up recommendations, please. -CERTIFIED READER IS NEEDED. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Figure 3. Read & analyse. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 9. No washing steps are required. Lysates are then transferred to the assay plate for the detection of total cRaf by HTRF. Step 5 Remove the plate sealer and read on an HTRF® compatible reader The blank control is used to check the Cryptate signal at 620 nm. • User-upgradable microplate reader in under 2 minutes • Array of detection cartridge applications at your fingertips. FOR HTRF CERTIFIED. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. HTRF is a proprietary time-resolved fluorescence technology that overcomesThe company offers different HTRF-certified, multidetection microplate readers such as the FLUOstar Omega, POLARstar Omega, CLARIOstar and PHERAstar. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. For more information. The kit includes everything you need: reagents, buffers and a microplate. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. It measures 96-well plates in less than 20 seconds and 1536-well formats in under one minute. Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. It can also be further streamlined toLysates are then transferred ®to the assay plate for the detection of Total TYK2 by HTRF reagents. FOR HTRF CERTIFIED READERThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Experimental details• HTRF®-Certified Reader **. 10 μL of GLP1 receptor cells were dispensed into wells of. The CLARIOstar® Plus is our most flexible multi-mode plate reader.